Description

Performs preprocessing and alignment of chromatin fastq files to fasta reference files using chromap.

Input

name:type
description
pattern

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

reads

:file

List of input FastQ files of size 1 and 2 for single-end and paired-end data, respectively.

meta2

:map

Groovy Map containing information for the fasta e.g. [ id:‘test’ ]

fasta

:file

Reference genome FASTA file

*.{fa,fasta,fna}

fai

:file

Reference genome FASTA index file

*.{fai}

meta3

:map

Groovy Map containing information for the index e.g. [ id:‘test’ ]

index

:file

Chromap genome index files (*.index)

barcodes

:file

Cell barcode files

whitelist

:file

Cell barcode whitelist file

chr_order

:file

Custom chromosome order

pairs_chr_order

:file

Natural chromosome order for pairs flipping

Output

name:type
description
pattern

bed

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.bed.gz

:file

BED file

*.bed.gz

bam

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.bam

:file

BAM file

*.bam

tagAlign

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.tagAlign.gz

:file

tagAlign file

*.tagAlign.gz

pairs

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.pairs.gz

:file

pairs file

*.pairs.gz

versions_chromap

${task.process}

:string

The name of the process

chromap

:string

The name of the tool

chromap --version 2>&1

:eval

The expression to obtain the version of the tool

versions_samtools

${task.process}

:string

The name of the process

samtools

:string

The name of the tool

samtools version | sed '1!d;s/.* //'

:eval

The expression to obtain the version of the tool

Topics

name:type
description
pattern

versions

${task.process}

:string

The name of the process

chromap

:string

The name of the tool

chromap --version 2>&1

:eval

The expression to obtain the version of the tool

${task.process}

:string

The name of the process

samtools

:string

The name of the tool

samtools version | sed '1!d;s/.* //'

:eval

The expression to obtain the version of the tool

Tools

chromap
GPL v3

Fast alignment and preprocessing of chromatin profiles